Question

RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when cutting DNA. 06. Restriction enzymes produce DNA fragments that are always of the same size. 7. DNA fragments of the same size move at different speeds through the gel. 58. DNA fragments with the same number of nucleotides(bases) will move through the gel at different speeds. 9. If a restriction enzyme made two cuts in a DNA molecule, four DNA fragments would be produced. 10. We would expect that the lambda DNA lane would only contain one fragment size if the DNA remained intact and was not degraded.

Answer 1

Q.1. Answer- B

Explanation- larger DNA fragments appear near the well and away from bottom because size of DNA is directly proportional to its movement in electric field. Larger the size then it will move slowly and smaller the size it will move faster in agarose gel.

Q.2. Answer- B

Explanation- larger DNA fragments move slowly through agarose gel because small DNA fragments move easily through pores of agarose gel but larger fragments can not.

Q.3. Answer- A

Explanation- DNA that has many restriction sites for a certain endonuclease will be cut into more fragments than the DNA with fewer restriction sites. DNA that has more restriction sites then the specific restriction endonuclease cleaves at that sites and we will get more fragments but if DNA has few sites then we will get few fragments as restriction sites are few and restriction enzymes are specific for cleavage at specific site.

Q.4. Answer- A

Explanation- If DNA is cut by using many restriction endonuclease then we will get many DNA fragments because DNA has many restriction sites for different endonuclease and by using many restriction enzymes DNA is cleaved at many sites and we will get more DNA fragments.