Question

1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments.

A. True

B. False

2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides.

A. true

B. false

3. Which of the following sequences are palindromes?

A. 5' TGGCCA 3'

B. 5' GAAAAG 3'

C. 5' CGATGG 3'

D. 5' GACGAC 3'

4. Below are the possible reasons for an incomplete DNA digestion (cleavage), except ______________.

A. insufficient restriction enzyme

B. incubation time too short

C. insufficient restriction sites

D. using the wrong buffer

5. An enzyme that recognizes a sequence of 5 base pairs long will cut a DNA on average every _____ bp.

A. 4096

B. 1024

C. 625

D. 20

6. Exonuclease is used to cut internal DNA molecules by hydrolyzes the phosphodiester.

A. True

B. Falso

7. What is the complementary DNA strand of the following sequence? ATGC

A. ATGC

B. CGTA

C. TACG

D. GCAT

8.____________ plot the locations cleaved by specific restriction enzymes on DNA molecules.

A. Gene maps

B. Restriction maps

C. Chromosomal attack maps

D. Digestion maps

9. Shorter DNA fragments will migrate through the gel ________________.

A. faster with lower voltage

B. slower than longer fragments

C. as fast as longer fragments

D. faster than longer fragments

10. The following sequence describes half of a palindromic restriction site. What is the complete sequence of restriction site? ATG_ _ _

A. ATG

B. CAT

C. TAC

D. GTA

Answer 1

1. True

See image below -

2. True

Smaller the size of restriction site, higher will be its distribution in the DNA and their for Greater will be its probability to be cut by a restriction enzyme.

This is because it is easier to find a combination of lesser number of bases as compared to a combination with more number of bases.

3. Palindromic types are those which are read same in both the directions, same from 5' to 3' and also from 3' to 5'.

4. Option C

The number of restriction site is variable and the performance of a restriction enzyme is not dependent upon the number of restriction site.

5. 1024

Location of restriction site of an enzyme which recognizes n number of bases = 4^n

For enzymes that recognizes 5 bases = 4^5 = 1024

6. False

Exonuclease cut terminal phosphodiester Bond whereas endonuclease cut internal phosphodiester bond.

7. Option C

Adenine pairs with thymine and guanine pairs with cytosine.

8. Option B

Restriction maps are made to show the location of restriction site on linear chromosome and plasmid. These maps help to determine the distance between two restriction sites.

9. Option D

Agarose gel electrophoresis is a technique which is used to separate protein mixture and nucleic acid mixture on the basis of their size and charge.

Agrose gel electrophoresis is used to separate nucleic acid fragments whereas sodium dodecyl sulphate polyacrylamide gel is used to separate protein fragments.

The Separation occurs by the application of external electric field. Smaller the fragment, faster it moves on the gel. Negatively charged fragment move towards the positively charged electrode.

10. Option B

Pallindromic sites will be read same in both the directions.

5' ATGCAT 3'

3' TACGTA 5'

I have answered all the questions with complete explaination.

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sita plasmid nut it 2

A5TGG CCA 3' 3 ACCGGTS 5'CGATGG3' 3'GCTA CCS 5GACGAC S 3'CTTCTG5 Pallindsome 5'GAAAA43 B) 3'CTTTTC5