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Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments:
4. Does your answer for part 3 help you in your effort to build a restriction map of the chromosome? Explain. Based on the fr


3. Propose a reason for why one or more chromosomal fragments might not be able to be cloned with the lab approach we used th
Experimental Overview: 1. Digest plasmid and chromosome with Hind III (pre-lab 1) 5....AVAGCTT...3 3..TTCGAVA...5 2. Ligate
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Answer #1

Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments:

From the gel picture, we can find the approximate size of each fragment by matching them with those in the 1 Kb ladder.

1. There are 7 possible Hind III fragments listed out of which 5 are found in the gel. The corresponding sizes of the fragments in the gel are: 9.4 kb ,6.5 kb, 2.3 kb, 2.0 kb and 564 bp.

2. The fragment sizes that are not found in the list would be the remaining two listed, 4.36 kb and 23 Kb.

4. Based on the explanation in answer 3, it is evident that the two Hind III fragments that are not jagged should be the end fragments of the linear chromosome, meaning, these two fragments would be flanking the 5 fragments in the gel. But, in order to build a restriction map, it is necessary to have at least 2 enzymes cutting the DNA so that we are able to determine the relative postions of the fragments.

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