Question

Based on the fragments cloned (and not cloned), draw the 45 kb hypothetical chromosome

The Cloning Problem Week 9 Write-up Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Sizes 1 Kb Ladder Possible Hind III Fragments: 10,000 8,000 564 bp 8;888 2.0 kb 2.3 kb 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb |||||||||| 23 kb 1,000 750 S00 250

Answer 1

Anshler: - from the above given gel picture, we -approximately can find the Each facagment Size by Matching with those in Ikbladdes. * The two Hind- lll faagments that are jagged Should be the End of fragments of the linear Chromosome. - It means in the gel these two fragments would be flaking the 5 fragments. - But, inades to a restriction map to - buid a have at least 2 Enzymes cutting the DNA Should necessary, so that we can able to the deteamination of the relative position of the fragments.