Question

Hi can someone help me understand part C and why the drawn in red lines are where they are.

Basically from the bp given how can I go back to cm so I can drawn them into the picture provided?

Do not need help with A or B.

The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 5. and the bands were visualized. The Hind III digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size 23130 9410 6680 4360 2320 2030 base pairs Using graph paper, calculate/deduce the following The sizes of the DNA fragments produced by enzyme "A" a. 20000, 19000, 13000, 500 b. which of the enzymes shown below was used in lane #2 of the gel (described as the unknown enzyme) Fragment sizes produced by Bam H1 Fragment sizes produced by EcoR1 Fragment sizes produced by Alw44 16841 7233 6770 6527 5626 5505 21226 7421 5804 5643 4878 3530 16179 13043 8286 5619 3375 2001 Alw44 Unknown Enzyme 9400 5.0 6600 4300 MEASUREMENTS 4 9. IN CM Hind III (BLUE) 23000 1600Q.0 0.1 13000 sboo nknown 1.5 2.3 2.44.0 20000 / 19фро 1 000 5.4 7.1 8.8 9.7 10.8 Draw bands on the gel (in lane X) where you would expect the fragments generated by digesting the circular plasmid (to the right) with restriction enzyme X (the cut sites are shown in parentheses and the total length of the plasmid is 20,000 b.p. long) c. X (120) X (18032) [Note: these bands are hypothetical ones and do not represent the true banding pattern of DNA cut with the given restriction enzymes.] (3200) X ( 2423 ) 1300, 2120, 3080, 7900, 5600 X (4531)

Answer 1

In the gel they given only the cm not the base pair. In Hind III they given 23000 bb can move upto 2.1cm
Same as 9400bp move up to 5cm. 6600bp move up to 6.2cm. Low base pair DNA moves to higher distance.

In unknown also you are getting relation ship between base pair and cm. Both Hind III and unknown cm has to
used as a reference for part C question.

In part C the base pair 7900 is compare to unknown 8000bp both are in 5.4 cm. SO red line drawn in 5.4cm form 7900 bp. All are drawn in approximately.

Next 5600 bp again seen in unknown exact 5600 bp are there at 7.1 cm. So drawn red line at 7.1 cm for 7900 bp.

For 3080 again compare to unknown 3300 bp it move up to 8.9 cm. So approximately red line drawn to 8.8 cm for 3080bp and so on.