Question

7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel

a. bottom to top; 5′ to 3′; shorter; early

b. top to bottom; 5′ to 3′; longer; early

c. bottom to top; 5′ to 3′; longer; later

d. top to bottom; 5′ to 3′; shorter; early

e. bottom to top; 3′ to 5′; shorter; early

8. Which technique amplifies DNA?

a. screening of the expression vectors

b. controlled termination of replication

c. polymerase cloning reaction

d. constructing a plasmid

e. polymerase chain reaction

9. Choose the means of generating diverse proteins from one gene. Select all that apply.

a. RNA methylation

b. mutations

c. RNA editing

d. alternative splicing

e. splicing

10. Which statement about CRISPR/Cas9 system is TRUE?

a. The CRISPR/Cas9 system is more efficient than the TALEN-based system because a single nuclease domain of Cas9 is enough to make a double-stranded nick.

b. The protospacer-adjacent motif has to be recognized by sg RNA to activate the NUC domain of Cas9.

c. sgRNA in cells is bound to the Cas9 protein to efficiently couple recognition and cleavage.

d. The target DNA site is recognized by the 3′ end of the single-guide RNA.

e. First, it was discovered in the genome of E. coli.

Answer 1

7. In a controlled termination method of DNA sequencing, reading the gel from bottom to top gives the sequence in the 5'to 3' direction; shorter fragments that were terminated early in polymerization move faster down the gel.

8. The technique that amplifies DNA is Polymerase Chain reaction.

9. The means of generating diverse proteins from one gene is Alternative splicing.

10. The true statement about CRISPR/Cas9 system is that the CRISPR/Cas9 system is more efficient than the TALEN-based system because a single nuclease domain of Cas9 is enough to make a double-stranded nick.