Question

Write true or false

______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being.

______ 2. As of 2009, all living human beings have had their entire genome sequenced.

______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C.

______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s.

______ 5. A restriction enzyme is useful because it is a generic enzyme that recognizes and cuts many different DNA sequences.

______ 6. Ligation of 2 DNA fragments is an enzyme-catalyzed reaction.

______ 7. Plasmids are circular, double-stranded DNA molecules.

______ 8. The gels used in gel electrophoresis are made of gelatin-like materials such as agarose or polyacrylamide.

______ 9. PCR stands for polyacrylamide gel electrophoresis.

______ 10. Using recombinant DNA techniques, scientists can join DNA fragments from different species.

______ 11. The process of DNA transfection is always 100% successful.

______ 12. A standard recombinant DNA procedure is to use antibiotics to kill off cells that have been successfully transfected with a recombinant plasmid.

______ 13. To determine if a DNA fragment has been successfully inserted into a plasmid, a scientist can sequence DNA of the plasmid.

______ 14. The standard procedure of gel electrophoresis separates DNA that is positively charged from DNA that is negatively charged.

______ 15. A chemical called ethidium bromide is often used to detect DNA that has been subjected to gel electrophoresis.

Answer 1

True True False. A, T, G, C - DNA: A, G, U, C - RNA True True These a produced by bacteria True Example ligase enzyme in DNA replication True seen in bacteria True False Polymerase chain Reaction True Desired genes can be joined False Not always False There are many applications of recombinant DNA True False all DNA are negatively charged True