Question

Q1) Which of these best describes the multiple cloning site (MCS)? (1 mark)

Select one:

a. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of DNA molecules.

b. A sequence of DNA which contains recognition sites for many restriction endonucleases.

c. A sequence of DNA used to identify and/or select for transformed cells.

d. A sequence of DNA which initiates replication and from which DNA replication proceeds.

e. A sequence of DNA which encodes a protein

Q2)Consider the following scenario: Imagine that you have been given a plasmid DNA sample and you are asked to determine if it is either pBCH2.0 or pBCH2.9IBM.

How would you answer this question using the restriction site mapping and fragment size information that you now have? Think about the different enzyme mixes (A, B, C, D) from your practical and justify which mix or enzyme would be able to distinguish between the two plasmids. (1 mark)

Answer 1

1. b. A sequence of DNA which contains recognition sites for many restriction endonucleases.
MCS or polylinker have lot of restriction site in a small region of DNA which used to insert a
foreign DNA into plasmid.

2. If two plasmid are cleaved by combination of restriction enzymes it gives different
fragment size in two different plasmids. Based on size variation in given plasmids we
identified which plasmid it is.