Question

Q1)

Which of these best describes the resistance marker? (1 mark)

Select one:

a. A sequence of DNA which encodes for a protein that gives the cells antibiotic resistance.

b. A sequence of DNA which initiates replication and from which DNA replication proceeds.

c. A sequence of DNA which contains recognition sites for many restriction endonucleases.

d. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of the DNA molecules

Q2)

Which of these best describes the origin of replication? (1 mark)

Select one:

a. A sequence of DNA which initiates replication and from which DNA replication proceeds.

b. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of DNA molecules.

c. A sequence of DNA which contains recognition sites for many restriction endonucleases.

d. A sequence of DNA which encodes for a protein.

e. A sequence of DNA used to identify and/or select for transformed cells.

Answer 1

Ans.1) (A) A sequence of DNA which encodes for a protein that gives the cells antibiotic resistance.

Explanation:-

resistance marker is a gene that produces a protein that provides cells expressing this protein with resistance to an antibiotic.

Ans.2)(A) A sequence of DNA which initiates replication and from which DNA replication proceeds.

Explanation:-

An origin of replication is a sequence of DNA at which replication is start. For small DNAs, including bacterial plasmids and small viruses, a single origin is sufficient but in eukaryotes DNAs have many origins, and DNA replication is initiated at all of them.