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15- Which option BEST describes sticky ends by restriction enzymes B. Sticky ends A. Sticky ends...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
help! 14. Which of the following factors does effect on DNA fragments separation during electrophoresis a....
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
Which of the following enzymes joins the paired sticky ends of DNA fragments? a. reverse transcriptase...
Which of the following enzymes joins the paired sticky ends of DNA fragments? a. reverse transcriptase b. restriction enzymes c. DNA ligase d. DNA polymerase e. helicase
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition &n
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c
Q1) Which of these best describes the resistance marker? (1 mark) Select one: a. A sequence...
Q1) Which of these best describes the resistance marker? (1 mark) Select one: a. A sequence of DNA which encodes for a protein that gives the cells antibiotic resistance. b. A sequence of DNA which initiates replication and from which DNA replication proceeds. c. A sequence of DNA which contains recognition sites for many restriction endonucleases. d. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of the DNA molecules Q2) Which of...
Q1) Which of these best describes the multiple cloning site (MCS)? (1 mark) Select one: a....
Q1) Which of these best describes the multiple cloning site (MCS)? (1 mark) Select one: a. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of DNA molecules. b. A sequence of DNA which contains recognition sites for many restriction endonucleases. c. A sequence of DNA used to identify and/or select for transformed cells. d. A sequence of DNA which initiates replication and from which DNA replication proceeds. e. A sequence of DNA...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
Chapter 10 Review: Biotechnology Learning Objectives and Application Questions Learning objectives are identified for you to...
Chapter 10 Review: Biotechnology Learning Objectives and Application Questions Learning objectives are identified for you to review your understanding of this topic. You are advised to reflect carefully on these objectives and check your own understanding to see if you have understood these essential concepts from this week’s reading. ANSWER THOROUGHLY all the application questions associated with each objective IN YOUR OWN WORDS. Learning Objective I: Evaluate the importance of biotechnology in modern societies (p. 228, 229) Application question #1...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
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