Which of the following properties is not necessary to make a good plasmid cloning vector? Select...
Which of the following properties is not necessary to make a good plasmid cloning vector? Select one:
a. Single recognition site for each restriction endonucleases to be used in cloning
b. High copy number
c. Selection marker
d. Fertility
e. Small size
Homework Answers
Answer :) option d
Restriction sites are important to insert a required sequence. A high copy number gives a high amount of replicated sequence. The selection marker is important to select the plasmid containing cells. Small size is preferable because it can carry the big size of an insert.
However, fertility is not a measure of having good plasmid cloning vector because bacteria can grow by binary fission and does not require fertility factor in the plasmid. Therefore, option d is the correct answer.
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Which of the following properties is not necessary to make a
good plasmid cloning vector? Select...
Q1) Which of these best describes the multiple cloning site (MCS)? (1 mark) Select one: a....
Q1) Which of these best describes the multiple cloning site (MCS)? (1 mark) Select one: a. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of DNA molecules. b. A sequence of DNA which contains recognition sites for many restriction endonucleases. c. A sequence of DNA used to identify and/or select for transformed cells. d. A sequence of DNA which initiates replication and from which DNA replication proceeds. e. A sequence of DNA...
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E....
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
Q1) Which of these best describes the resistance marker? (1 mark) Select one: a. A sequence...
Q1) Which of these best describes the resistance marker? (1 mark) Select one: a. A sequence of DNA which encodes for a protein that gives the cells antibiotic resistance. b. A sequence of DNA which initiates replication and from which DNA replication proceeds. c. A sequence of DNA which contains recognition sites for many restriction endonucleases. d. A sequence of DNA which can be used in agarose gel electrophoresis to determine the size of the DNA molecules Q2) Which of...
Q1) What is the size of the entire pOTC-Δ plasmid (in units of base pairs, bp)?...
Q1)
What is the size of the entire pOTC-Δ plasmid (in units of base
pairs, bp)? Simply input one NUMBER with no
spaces, punctuation or units. Make sure you write the
number in digits not words (1 mark)
Q2)
What are the selection markers for the pOTC-Δ plasmid? (1
mark)
Select one:
a. Hygromycin resistance gene
b. NdeI
c. NdeI and KpnI
d. Ampicillin resistance gene
e. Ampicillin resistance gene and the hygromycin resistance
gene
f. pUC Ori and OTC-Δ...
R-plasmids have which ONE of the following properties: A. Are less than 10 kb in size...
R-plasmids have which ONE of the following properties: A. Are less than 10 kb in size B. Contain antibiotic resistance genes and are self-transmissible C. Have a high copy number D. Are mobilizable but not self-transmissible E. Are commonly used for cloning recombinant DNA
Cloning 2 Below is the restriction map of a 10 kb piece of DNA. Also shown...
Cloning 2
Below is the restriction map of a 10 kb piece of DNA. Also shown
below is a cloning vector which has two unique restriction enzyme
recognition sites, one for EcoRI (E) and one for HindIII (H). The
location of the kanamycin (kan) and ampicillin (amp) resistance
genes is also shown. Kanamycin and ampicillin are antibiotics that
are commonly used to select transformed E. colicells (consult the
Lab Manual for more information). Note that the HindIII site is
located...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a)...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
Q1)
What is the size of the entire pOTC-Δ plasmid (in units of base
pairs, bp)? Simply input one NUMBER with no
spaces, punctuation or units. Make sure you write the
number in digits not words (1 mark)
Q2)
What are the selection markers for the pOTC-Δ plasmid? (1
mark)
Select one:
a. Hygromycin resistance gene
b. NdeI
c. NdeI and KpnI
d. Ampicillin resistance gene
e. Ampicillin resistance gene and the hygromycin resistance
gene
f. pUC Ori and OTC-Δ...
Cloning 2
Below is the restriction map of a 10 kb piece of DNA. Also shown
below is a cloning vector which has two unique restriction enzyme
recognition sites, one for EcoRI (E) and one for HindIII (H). The
location of the kanamycin (kan) and ampicillin (amp) resistance
genes is also shown. Kanamycin and ampicillin are antibiotics that
are commonly used to select transformed E. colicells (consult the
Lab Manual for more information). Note that the HindIII site is
located...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
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