Question

Shown below is a map of the OTC-A plasmid which you purified last week from Ecolicells. The positions of the recognition sites for a number of restriction endonucleases are shown (in units of base pairs, 'bp') as are the positions of the genes encoding the proteins that confer resistance to the antibiotics ampicillin and hygromycin. The site initiating replication and the region of the DNA encoding the OTC-A protein are also indicated. Nhel Kpni 1569 1601 Kpl 1030 2295 988 Noti 2310 Sphi 2559 Ndet POTC-A 6401 bp Hygro Amp 1 ZpUC Ori COR 52604 587 Amp: Ampicillin resistance 5405-6265 Hygro: Hygromycin resistance 2933 - 3943

Q1)

What is the size of the entire pOTC-Δ plasmid (in units of base pairs, bp)? Simply input one NUMBER with no spaces, punctuation or units. Make sure you write the number in digits not words (1 mark)

Q2)

What are the selection markers for the pOTC-Δ plasmid? (1 mark)

Select one:

a. Hygromycin resistance gene

b. NdeI

c. NdeI and KpnI

d. Ampicillin resistance gene

e. Ampicillin resistance gene and the hygromycin resistance gene

f. pUC Ori and OTC-Δ

Answer 1

Q-1)

The size of the plasmid is 6401 bp

Q-2)

ampicillin and hygromycin resistance genes are the selectable markers.