Question

CHICOU COUPLUNDU Nhel Kpn 1 1569 1601 Not 1 KpnI 1030 988 2295/ 2310 ОТСА - Sphl 2559 Nde1 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405-6265 Hygro: Hygromycin resistance gene 12933 - 3943

How can ampicilin resistance gene and the hygromycin resistance gene help us to select for bacterial cells containing the pOTC- delta plasmid?

Answer 1

For selecting bacterial cells containing pOTC-delta plasmid one can use its ampicillin and hygromycin resistance gene.

for the same, one needs to grow the bacterial culture in a media containing both ampicillin and hygromycin. In such a case, only the transformed bacterial would be having resistance for these 2 antibiotics and would be able to survive and grow in the media.