Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help...
Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help us to select for bacterial cells containing the pOTC-Δ plasmid? (1 mark )
Homework Answers
As the pOTC-Δ plasmid carry selection marker genes i.e ampicillin and hygromycin resistance genes, the bacteria which carry the plasmid will be resistant to these antibiotics and can grow in medium which contain these two antibiotics. Te baccterial cells which does not contain the plasmid pOTC-Δ will die in the medium which contains either ampicilin, or hygromycin or both.
Add Answer to:
Q1) How can these selection markers ( Ampicillin resistance gene
and the Hygromycin resistance gene ) help...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
How can ampicilin resistance gene and the hygromycin resistance gene help us to select for bacterial...
How can ampicilin resistance gene and the hygromycin
resistance gene help us to select for bacterial cells containing
the pOTC- delta plasmid?
CHICOU COUPLUNDU Nhel Kpn 1 1569 1601 Not 1 KpnI 1030 988 2295/ 2310 ОТСА - Sphl 2559 Nde1 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405-6265 Hygro: Hygromycin resistance gene 12933 - 3943
Q1) What is the size of the entire pOTC-Δ plasmid (in units of base pairs, bp)?...
Q1)
What is the size of the entire pOTC-Δ plasmid (in units of base
pairs, bp)? Simply input one NUMBER with no
spaces, punctuation or units. Make sure you write the
number in digits not words (1 mark)
Q2)
What are the selection markers for the pOTC-Δ plasmid? (1
mark)
Select one:
a. Hygromycin resistance gene
b. NdeI
c. NdeI and KpnI
d. Ampicillin resistance gene
e. Ampicillin resistance gene and the hygromycin resistance
gene
f. pUC Ori and OTC-Δ...
hi I need help with these questions please q1 How long is the DNA sequence encoding...
hi I need help with these questions please
q1 How long is the DNA sequence encoding the protein that
confers ampicillin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q2 How long is the DNA sequence encoding the protein that
confers hygromycin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q3 What is the size of the OTC-Δ DNA sequence that has been
inserted into the...
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid that has a gene for ampicillin resistance and then plated on medium containing ampicillin. What will happen next? 1.Only E. coli cells that have not taken up a plasmid will grow and form colonies. 2.only E. coli cells that have taken up a plasmid will grow and form colonies. 3.E. coli cells with and without a plasmid will grow and form colonies. 4.The plasmid...
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you...
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you try to deactivate it by transformation. What would be a selective medium to check if this deactivation was well done? 1. Rich medium + ampicillin 2. Rich medium - ampicillin B) Considering question A and if the deactivation went well, in which case (s) will the transformed bacterial cells be alive? 1. Rich medium + ampicillin 2- Rich medium - ampicillin
Prac result Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д...
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
How can ampicilin resistance gene and the hygromycin
resistance gene help us to select for bacterial cells containing
the pOTC- delta plasmid?
CHICOU COUPLUNDU Nhel Kpn 1 1569 1601 Not 1 KpnI 1030 988 2295/ 2310 ОТСА - Sphl 2559 Nde1 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405-6265 Hygro: Hygromycin resistance gene 12933 - 3943
Q1)
What is the size of the entire pOTC-Δ plasmid (in units of base
pairs, bp)? Simply input one NUMBER with no
spaces, punctuation or units. Make sure you write the
number in digits not words (1 mark)
Q2)
What are the selection markers for the pOTC-Δ plasmid? (1
mark)
Select one:
a. Hygromycin resistance gene
b. NdeI
c. NdeI and KpnI
d. Ampicillin resistance gene
e. Ampicillin resistance gene and the hygromycin resistance
gene
f. pUC Ori and OTC-Δ...
hi I need help with these questions please
q1 How long is the DNA sequence encoding the protein that
confers ampicillin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q2 How long is the DNA sequence encoding the protein that
confers hygromycin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q3 What is the size of the OTC-Δ DNA sequence that has been
inserted into the...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you try to deactivate it by transformation. What would be a selective medium to check if this deactivation was well done? 1. Rich medium + ampicillin 2. Rich medium - ampicillin B) Considering question A and if the deactivation went well, in which case (s) will the transformed bacterial cells be alive? 1. Rich medium + ampicillin 2- Rich medium - ampicillin
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
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