E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid that has a gene for ampicillin resistance and then plated on medium containing ampicillin. What will happen next?
1.Only E. coli cells that have not taken up a plasmid will grow and form colonies.
2.only E. coli cells that have taken up a plasmid will grow and form colonies.
3.E. coli cells with and without a plasmid will grow and form colonies.
4.The plasmid is resistant to ampicillin, so it will survive and replicate outside of the bacterial cells.
Homework Answers
E.coli cells will take up the plasmid cells and will become resistant. This process is called transformation. Those E.coli cells who have taken up a plasmid will grow and form colonies. So option B is correct.
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E. coli cells that are sensitive to ampicillin are mixed with
many copies of a plasmid...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with a plasmid that conta ampiellin-resistance gene plus a gene of interest, c.g. the gene for the female hormone, estrogen coll is placed on a growth medium containing ampicillin, then a. no bacteria will grow. b, only ampicillin-sensitive bacteria will grow. c. only bacteria containing the plasmid will grow. d. all bacteria will grow. c. none of these the flavinanses except
Please answer these four questions. 6C. A type of plasmid used frequently as a vector in...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and is ampicillin sensitive with 109 cells of strain B which requires phenylalanine and tyrosine and is ampicillin resistant. In order to detect whether genetic recombination had occurred between the two strains it would be best to grow the mixture of cells in A) minimal glucose medium plus ampicillin B) minimal glucose medium with no supplements C) minimal glucose medium plus lysine and phenylalanine D)...
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E....
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
cuse u grunt pleyenire bullial cell 3) Suppose that you place some bacterial cells on an...
cuse u grunt pleyenire bullial cell 3) Suppose that you place some bacterial cells on an LB agar plate containing ampicillin. One of these bacterial cells is resistant to ampicillin; the other bacterial cells are not resistant to ampicillin (see far left plate on the picture below). After incubating the plate for 24 hours, only the ampicillin-resistant cell is able to form a colony. However, if the plate is incubated for an additional 48 hours, the other nearby bacterial cells...
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli....
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli. As they are designing their experimental protocols they are concerned that they will not be able to identify colonies which were successfully transformed, with the correct plasmid/insertion construct. Provide a suggested mechanism or method to address their concerns. Specifically answer the following two questions and provide details on the biological mechanism underpinning the method. a. Describe a mechanism to determine if the bacterial have...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with a plasmid that conta ampiellin-resistance gene plus a gene of interest, c.g. the gene for the female hormone, estrogen coll is placed on a growth medium containing ampicillin, then a. no bacteria will grow. b, only ampicillin-sensitive bacteria will grow. c. only bacteria containing the plasmid will grow. d. all bacteria will grow. c. none of these the flavinanses except
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
cuse u grunt pleyenire bullial cell 3) Suppose that you place some bacterial cells on an LB agar plate containing ampicillin. One of these bacterial cells is resistant to ampicillin; the other bacterial cells are not resistant to ampicillin (see far left plate on the picture below). After incubating the plate for 24 hours, only the ampicillin-resistant cell is able to form a colony. However, if the plate is incubated for an additional 48 hours, the other nearby bacterial cells...
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