Question

Which statement about gene cloning is false?

A. Bacterial cells take up recombinant plasmids by transformation.

B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector.

C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes

D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.

Answer 1

To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes statement about gene cloning is false

(to insert a gene of interest in to a vector, the gene of interest and vector must be digest with same restriction enzyme to produce same/ complementary sticky ends which allow to ligate gene of interest and vector to each other, if vector and gene are digest with different restriction enzymes then it will create different sticky ends and there is no ligation and recombination of DNA molecule.)