Question

1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria C 0.5 points QUESTION 3 1. In nature, transformation is completed when bacteria uptake a foreign DNA. foreign DNA molecules enters bacteria and recombine with bacterial genome C foreign DNA enters bacteria through bacteriophage bacterium receives DNA fragment from sex pilus of donor bacterium

Answer 1

Q1) pGLO plasmid

• Here GPF Gene is the gene of interest.
• E.coli Cell is the host cell.
• pGLO plasmid carreis the gene of interest so it is  the vector. plasmids were the vectors.
• ampicillin treated agar medium is a growth medium of bacteria without contamination.

Q2) Breakdown the ampicillin

• bla gene is the antibiotic resistant gene so it may bread the antibiotic ampicillin.
• polymerase enzymes were responsible for the transcription in DNA/ RNA .so same here in GFP gene.
• green fluroscent Protein (GFP) has the fluroscence trait .so bla gene may not express the fluroscent triat in bacteria.
• it does not contain any uptake of DNA molecule.

Q3) bacteria receives DNA fragment from sex pilus of donor bacterium

• In transformation the DNA molecule of one bacteria is uptaken by other bacteria through molecular biology exprement
• where as the foreign DNA molecule enters bacteria and recombine with bacterial genome is called as recombination.
• whese as is bacterophage the Virus and bacteria combines and transfer the DNA.
• pili is a structure by which the bacteria transfer the DNA fragments to the other bacteria its a natural process