Question

The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts

Answer 1

Ans 4: Bacteria did not receive a plasmid carrying the antibiotic resistant gene hence these cells will not grow on the LB plate.

Hence option B is correct.

5: The green fluorescent protein is extremely hydrophobic compared to host bacterial proteins, which are relatively hydrophilic. This allows the use of hydrophobic chromatography for the purification of recombinant proteins containing green fluorescent protein as a tag.

Hence option D is correct.

6: The concentration is 80 nanogram/microliter so 15 microliter has 1200 nanograms which is equal to 1.2 microgram.

Hence option C is correct.

11: I am not sure about this question.

12: Supernatant is the usually clear liquid overlying material deposited by settling, precipitation, or centrifugation.

13: In DNA electrophoresis, buffers like TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) are used most commonly.

Hence option B is correct.

20:  Buffer P2 is a lysis buffer solution produced by Qiagen. It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and 200mM NaOH. It is used in conjunction with other resuspension buffers and lysis buffers to release DNA from cells, often as part of the alkaline lysis method of purifying plasmid DNA from bacterial cell culture.

Hence option A is correct.

25: The cell is containing 1% solutes is placed into a solution of pure distilled water, so the cell is hypertonic and the solution of pure distilled water is hypotonic. Osmosis is the movement of a solvent across a semipermeable membrane toward a higher concentration of solute (lower concentration of solvent).

Hence the net movement of water through the cell movement into the cell.