Question

Wullugard to GFP and bla, what are the genotypes of E. coli before and after transformation? 2. What was the purpose of transferring the +DNA and DNA tube from ice to hot water to ice? 3. Why were the tubes incubated at room temperature for 10 minutes before spreading their contents onto the agar plates? Why not just inoculate the plates right after the heat shock?

Answer 1

The coli bacteria are contagious while the others are not,depending on the E.Coli strain and the infection type.Some strains that cause gastrointestinal problems can be transmitted person to person.Usually they are transfered oraly and even indirectly by contaminated food or water.THis bacterium is very crucial in modern biotechnology.As the scientist use them to store DNA sequence from other organismto produce protein and to test protein function.

1 E. coli - fullform Escherichia coli. This is a type of bacteria that normally lived in love your intestines. It is also found in the Gut of some animals. Most type of E-coli are harmless and even keep our digestive tract healthier. GFP = Green Ffluroscent Protein Oblained from Gellyfish which giver geen fluroscence cohen exposed to light which has 238 aminoacid. BhA = Biologics license application. Genotype of E-Coli before and after transformation are as follows: The purpose of transformation of cal is to introduce a foregn plasmid into bacteria so that the bacteria then Complifies the plasmid making looge quantities of it. In the process, PGREEN plasmid contain a mutant Vession of app linked to another gene called bela galactosidase Genotype of an E-coli strain is an important aspect of competent cous in that it determine whether the cells can be yown on speagic media, whether trey may be bed for transformation with spege DNA typer and to hether they are appropriate for certain cloning Stockgles commonly wed Streins are ARIZO, ASI, BNNlOdo C600, PHI etc.