Question 1
- Already all the items are correctly matched
Question 2
- heat shock
QUESTION 1 Match each item with its function in our transformation experiment. (5 points) - Makes...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation occurred on which agar plate (s)? What evidence do you have that the bacteria were transformed here? 3. Which plates have glowing growth? Explain what causes bacteria to glow. II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
Need help filling in the chart and answering the questions that go along with it. I have added the procedure and the instructions as well as the "results" that are supposed to be used to fill in the chart. Thank you! We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
please help QUESTION 1 When transforming bacterial cells, a researcher is interested in generating bacteria that exogenously express the gene that encodes GFP (green fluorescent protein). After transformation, she finds that no bacteria colonies grew on her selective media. Which of the following best explains the results of her transformation protocol? The concentration of the antibiotics in the selection media was too low, therefore no bacteria grew. Her bacterial cells were not competent so they took up excessive amounts of...