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QUESTION 1 When transforming bacterial cells, a researcher is interested in generating bacteria that exogenously express the gene that encodes GFP (green fluorescent protein). After transformation, she finds that no bacteria colonies grew on her selective media. Which of the following best explains the results of her transformation protocol? The concentration of the antibiotics in the selection media was too low, therefore no bacteria grew. Her bacterial cells were not competent so they took up excessive amounts of the GFP-containing plasmid. She forgot the heat shock step, so the DNA was not forced into bacterial cells. The plasmid that she used contained a sequence that encoded antibacterial resistance. QUESTION 2 A researcher is studying DNA replication in a few new organisms. During this process, she notes multiple origins of replication, in addition to "overhanging regions on the ends of linear chromosomes, which are extended by an enzyme. The organisms are likely: A. prokaryotes B. eukaryotes C. both a and b D. neither a orb QUESTION 3 Click Save and Submit to save and submit. Click Save All Answers to see all ansas Save All Answers

Answer 1

Answer-

According to the given question-

Green fluorescent protein - a protein made up of 238 amino acid molecules and it turn bright green color when blue or ultraviolet light fall on it. GFP without any cofactors, and gene products form the internal chromophore and used as a reporter for expression of several genes , used for making biosensors. GFP can be easily introduced and maintained by using transgenic techniques, in bacteria, yeasts, fungi, fish and mammals.

Transformation is a process in which bacteria take up the foreign desired DNA by restriction digest and ligation process used in DNA cloning. Bacteria those have this certain type of plasmid are made to antibiotic-resistant, and can easily grow on the plate in the form of colony.

(1) so considering above information the correct answer for the first question is She forget the heat shock step, so the DNA was not forced into in the bacterial cells because this step is used for the insertion of constructed plasmid to insert into bacterial cells.

(2) DNA Replication- process by which two similar copies of DNA produced from a double-stranded DNA.

Origin of replication or the  replication origin or ORI is a specific sequence present in a genome where the process of replication is started. During initiation of DNA Replication a special type of  proteins bind with the ORI after that the enzyme  helicase breaks the DNA helix and unwind the DNA Strand so that a structure called replication forks at the origin of replication.

the correct answer for the first question is Eukaryotes have mutliple origin of replication because the size of eukaryotic chromosomes are large due to which multiple ORI is required for the replication of DNA in in very short period of time.