Question

The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with a linearized plasmid without a DNA insert ligated in, as a means of amplifying the plasmid vector. This ensures that the plasmid is long enough to be stably replicated. Next →

Answer 1

this enables the selecting a bacteria colony transformed with a plasmid with a DNA insert ligated as a means of amplifying a specified piece of DNA .

only the transformed colonies will survive which have the ampillicin resistant gene which can degrade the ampillicin present in the media . ( These colonies have the gene of interest).

non transformed will not be able to survive due to lack of ampillicin resistant gene. ampillicin inhibits the enzyme in bacteria which is involve in bacterial cell wall synthesis.