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Why is uncut plasmid used as a positive control for a transformation experiment?  If a researcher saw...

Why is uncut plasmid used as a positive control for a transformation experiment?  If a researcher saw no colonies on a positive control plate, what are some possible causes?  Think specifically about the transformation protocol itself.  Assume the plasmid DNA was high quality and at a sufficiently high concentration that one would expect many transformants on the plate.  

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Positive control is a measure of efficiency of transformation. It also testo I the efficiency of transformation protocol. So7 Excessive of competent be avoided . transformation freezing and thawing cells I should .. This reduces efficiency. Seamtech

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