Question

You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem?

A. Random mutation has inactivated the antibiotic resistance genes

B. Plasmid incompatibility will not allow both plasmids to persist

C. E. coli cannot maintain two plasmids

D. The plasmid(s) have integrated into the bacterial chromosome

E. A phage has neutralized one of the plasmids

F. None of the above is correct

Answer 1

Option B is right. Two different plasmid can't coexist in a bacterial cell due to incompatibility in the sequence . Replication machinery always replicate one plasmid . By the time frame one type plasmid going to be out of the cell.

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