The Cas9 plasmid contains a kanamycin resistance gene and the gRNA plasmid contains an ampicillin resistance...
The Cas9 plasmid contains a kanamycin resistance gene and the gRNA plasmid contains an ampicillin resistance gene. What was the purpose of adding these genes to the plasmids during the CRISPER procedure?
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The Cas9 plasmid contains a kanamycin resistance gene and the
gRNA plasmid contains an ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
What part of the pGlo plasmid is inducible a) the genes for ampicillin resistance b) the...
What part of the pGlo plasmid is inducible a) the genes for ampicillin resistance b) the genes to break down arabinose c) the genes to form a pilus d) all the genes are inducible
Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help...
Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help us to select for bacterial cells containing the pOTC-Δ plasmid? (1 mark )
What is the danger of using an ampicillin resistance gene as part of a bacterial transformation?...
What is the danger of using an ampicillin resistance gene as part of a bacterial transformation? What could happen if this gene was spread to wild bacterial population? What are four ways the ampicillin resistance genes could be prevented from escaping to the wild. Briefly explain each method of prevention.
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B...
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B (5.7 kb) ECORI Kpnl BamHI - Xmal Plasmid A (4.2 kb) Ampicillin resistance Kanamycin resistance 15. Outline the strategy for how you would clone the GFP cassette from plasmid A into plasmid B? Please include descriptions of how you would select for potentially correct clones and verify the final construct. For the purposes of this exercise, assume that sequencing services are not available. *...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid that has a gene for ampicillin resistance and then plated on medium containing ampicillin. What will happen next? 1.Only E. coli cells that have not taken up a plasmid will grow and form colonies. 2.only E. coli cells that have taken up a plasmid will grow and form colonies. 3.E. coli cells with and without a plasmid will grow and form colonies. 4.The plasmid...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B (5.7 kb) ECORI Kpnl BamHI - Xmal Plasmid A (4.2 kb) Ampicillin resistance Kanamycin resistance 15. Outline the strategy for how you would clone the GFP cassette from plasmid A into plasmid B? Please include descriptions of how you would select for potentially correct clones and verify the final construct. For the purposes of this exercise, assume that sequencing services are not available. *...
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