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What part of the pGlo plasmid is inducible a) the genes for ampicillin resistance b) the...

What part of the pGlo plasmid is inducible

a) the genes for ampicillin resistance

b) the genes to break down arabinose

c) the genes to form a pilus

d) all the genes are inducible

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Answer #1

option B is the correct answer.

The plasmid naturally contains an operon for arabinose digestion.If arabinose is present in the nutrient medium,the operon's structural genes for arabinose digestion will be turned on.Thus when arabinose is present to turn on the arabinose operon,GFP is produced and the bacteria can fluoresce.The pGLO plasmid contains a origin of replication,a selectable marker, and the gene for green fluorescent protein,The pGLO plasmid was engineered to allow inducible expression of GFP.Inducible means only when the cells will produce GFP,When an inducer is present.In this case the inducer is arabinose.pGLO plasmid contains the gene betalactamase,which provides resistance for ampicillin.Only transformed bacteria that contain the plasmid and express betalactamase can grow o plates that contain amipicillin.

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