Question

You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for REP After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an overnight incubation at 37°C, what would you predict the bacteria

Answer 1

pGLO plasmid contains the gene for RFP and a gene for resistance to the antibiotic ampicillin. Transfomed bacteria will grow on LB plate containing ampicilin and arabinose. The pGLO also incorporates a special gene regulation system that can be used to control expression of the fluorescent protein in transformed cells. The transformed cells will be red color.