Question

D7. Looking at your staging results, which phase of the cell cycle does the cell spend the most time in? interphase prophase metaphase anaphase telaphase cytokinesis 8. if we were using slides from a frog instead of an onion to do this lab, which cell type do you think would be best for us to view mitosis? frog skin frog embryo frog brain frog muscle D5. You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so, you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for RFP. After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP, you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an overnight incubation at 37°C, what would you predict the bacteria would glow green under UV light. would glow red under UV light would not glow under UV light, because the protein is not GFP. Impossible to predict.

Answer 1

Ans 1- interphase

In mitosis, the Interphase is the growth phase of a cell in the cycle. The eukaryotic cell spends maximum time in interphase part of the cell cycle the cell spends around 90% of its time in interphase.

Answer 2 - Frog Embryo

Embryos from the frog have been widely used in developmental biology and study the various body systems. The division in these cells is fast as compared to the cells in other parts. Isolation of embryo cells is also easy hence they can ve used to study cell division.

Answer 3 - Would not glow under UV because the protein is not GFP

Red fluorescent protein (RFP) is a protein that can be visualized optimally at 588 nm but UV range is 100–400 nm hence it cannot be detected under UV.