Homework Answers
pGLO plasmid contains the bla gene which is responsible for the ampicillin resistance this gene is always expressed.
the GFP is cloned under the arabinose promoter, it is expressed only in the presence of Arabinose, AraC gene codes for the regulatory protein, which controls the expression of the arabinose operon, AraC can inhibit the expression of the Arabinose operon in the absence of arabinose, so the GFP is expressed only in the presence of arabinose.
7) a) ampr ( or bla) ampicillin resistance due to the expression of beta-lactamase
8) b) GFP ( green fluorescent protein)
the enzymes for the arabinose metabolism are not present in the plasmid.
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Question 7 070.5 pts Which gene(s) on the PGLO plasmid are always turned...
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein...
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for REP After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
QUESTION 1 Match each item with its function in our transformation experiment. (5 points) - Makes...
QUESTION 1 Match each item with its function in our transformation experiment. (5 points) - Makes cells permeable to plasmid DNA A. CaCl2 - Selects for the growth of transformed cells B. Ampicillin - Provides the GFP gene C. PGLO plasmid • Induces GFP gene expression D. Arabinose - Causes Green Fluorescent Protein to fluoresce E. UV Light QUESTION 2 To increase the efficiency of transformation, cells will be placed in a 42°C water bath for 50 seconds during which...
D7. Looking at your staging results, which phase of the cell cycle does the cell spend...
D7. Looking at your staging results, which phase of the cell cycle does the cell spend the most time in? interphase prophase metaphase anaphase telaphase cytokinesis 8. if we were using slides from a frog instead of an onion to do this lab, which cell type do you think would be best for us to view mitosis? frog skin frog embryo frog brain frog muscle D5. You want to use PGLO to produce red fluorescent protein (RFP) instead of green...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for REP After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
QUESTION 1 Match each item with its function in our transformation experiment. (5 points) - Makes cells permeable to plasmid DNA A. CaCl2 - Selects for the growth of transformed cells B. Ampicillin - Provides the GFP gene C. PGLO plasmid • Induces GFP gene expression D. Arabinose - Causes Green Fluorescent Protein to fluoresce E. UV Light QUESTION 2 To increase the efficiency of transformation, cells will be placed in a 42°C water bath for 50 seconds during which...
D7. Looking at your staging results, which phase of the cell cycle does the cell spend the most time in? interphase prophase metaphase anaphase telaphase cytokinesis 8. if we were using slides from a frog instead of an onion to do this lab, which cell type do you think would be best for us to view mitosis? frog skin frog embryo frog brain frog muscle D5. You want to use PGLO to produce red fluorescent protein (RFP) instead of green...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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