Homework Answers
a. The function of gene is selection. The cells which have taken up the gene during the transformation will survive when ampicllin is added to the media for the growth of bacteria. The cells which have not taken up the gene during transformation will not survive. The presence of any antibiotic resistant gene helps as a marker of selection. So, if the ampicillin resistance gene is inserted it will help in selection.
b. When methicillin is added to the plate after cloning all the cells will grow. Colonies will be formed. In the sketch a circular plate will show many colonies When ampicillin is added to the media cells which have been transformed with the amplicillin resistant gene only they will grow, i.e colonies will be formed. In the sketch a circular plate will show some colonies but fewer than the previous.
Add Answer to:
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E....
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S...
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S pts.). The gene fragment of interest was obtained by digestion of chromosomal DNA with the restriction enzyme Sall and subsequent purification using agarose gel electrophoresis. Which antiblotic would you use in the final step of the cloning procedure, and Pst why? EcoR Sal Ampicillin Tetracycline resistanica(Ter Amp) PBR322 4,361 bp) Origin of replicatiorn (ori Pvull 8. Assume that your gene fragment from question...
4. The DNA profiles to the right below represent four different individuals. o 111 Which of...
4. The DNA profiles to the right below represent four different individuals. o 111 Which of the following statements is consistent with the results? A. B is the child of A and C. B. C is the child of A and B. C. D is the child of B and C. D. A is the child of B and C. E. A is the child of C and D. <ULTE ILI UTILIT THITI TIL ILI 5. A eukaryotic gene with...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S pts.). The gene fragment of interest was obtained by digestion of chromosomal DNA with the restriction enzyme Sall and subsequent purification using agarose gel electrophoresis. Which antiblotic would you use in the final step of the cloning procedure, and Pst why? EcoR Sal Ampicillin Tetracycline resistanica(Ter Amp) PBR322 4,361 bp) Origin of replicatiorn (ori Pvull 8. Assume that your gene fragment from question...
4. The DNA profiles to the right below represent four different individuals. o 111 Which of the following statements is consistent with the results? A. B is the child of A and C. B. C is the child of A and B. C. D is the child of B and C. D. A is the child of B and C. E. A is the child of C and D. <ULTE ILI UTILIT THITI TIL ILI 5. A eukaryotic gene with...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Most questions answered within 3 hours.
-
Suppose that you are an official with Mexico's economic
development agency. Write a one-page memo detailing...
asked 6 minutes ago -
If you were an international firm, why would you support the
concept of global free trade?...
asked 22 minutes ago -
Cisco packet tracer
Q1) Do you get any changes of IP address when packet is
traversing...
asked 1 hour ago -
What is the pressure inside a 33.0 L container holding 106.4 kg
of argon gas at...
asked 1 hour ago -
Question no 2
A housekeeping support department budgets its costs at
SR 40,000 per month plus...
asked 1 hour ago -
A 1400Kg sports car accelerates from rest to 90km/h in 7.0s.
What is the average power...
asked 2 hours ago -
For the following reaction, 0.128 moles of
potassium hydrogen sulfateare mixed with
0.504 moles of potassium...
asked 6 hours ago -
1. What is the present value of $400, three years in the future
if the interest...
asked 6 hours ago -
The labor force minus the number of employed equals the number
of unemployed.
a. True
b....
asked 8 hours ago -
Determine the mass in units of grams​ [g] of 0.49 moles​ [mol]
of a new fictitious...
asked 9 hours ago -
A horizontal mass of M=5kg is on a spring and stretched to
x=0.5m when released from...
asked 10 hours ago -
26 of 50
"I have worked at the Arizona Humane Society for ten years, and
have...
asked 10 hours ago