Homework Answers
AnsA) : 1. Rich Medium+ Ampicillin
- We will use LB (Luria Bertani) Broth having a suitable amount of Ampicillin solution; say for eg. 0.05 gram ampicillin dissolved in 1ml sterile distilled water to grow the already Ampicillin resistant bacteria and keep it for incubation at 37°C for 24 hrs.
If the deactivation has been occurred in the bacteria it will no longer remain ampicillin resistant. Hence the day after incubation you will see no growth of the bacteria in the ampicillin mixed rich medium.
AnsB) : 2) Rich Medium- Ampicillin
- If the deactivation has been occurred the newly transformed cells will now lack the selectable marker of Ampicillin, hence it will no longer grow in the Ampicillin rich media and will surely grow in the absence of Ampicillin.
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A) You are interested in a gene coding for ampicillin resistance at a bacteria and you...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
please help QUESTION 1 When transforming bacterial cells, a researcher is interested in generating bacteria that...
please help
QUESTION 1 When transforming bacterial cells, a researcher is interested in generating bacteria that exogenously express the gene that encodes GFP (green fluorescent protein). After transformation, she finds that no bacteria colonies grew on her selective media. Which of the following best explains the results of her transformation protocol? The concentration of the antibiotics in the selection media was too low, therefore no bacteria grew. Her bacterial cells were not competent so they took up excessive amounts of...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
please help
QUESTION 1 When transforming bacterial cells, a researcher is interested in generating bacteria that exogenously express the gene that encodes GFP (green fluorescent protein). After transformation, she finds that no bacteria colonies grew on her selective media. Which of the following best explains the results of her transformation protocol? The concentration of the antibiotics in the selection media was too low, therefore no bacteria grew. Her bacterial cells were not competent so they took up excessive amounts of...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
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