Question

command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4. Cloning vectors have three essential components that make up their sequence. Which one is NOT one of those three? a) origin of replication c) polyA region b) dominant selectable marker d) polylinker region 5. Oligo (dT) and reverse transcriptase are used when making: a) a genomic library c) a restriction endonuclease map b) a cDNA library d) a DNA sequencing reaction 6. 2,3-Dideoxyribonucleoside triphophates ("poisoned dNTPs) are used when making: a) a genomic library c) a restriction endonuclease map b) a cDNA library d) a DNA sequencing reaction 7. What type of hybridization results from running genomic DNA on an agarose gel, transferring to a membrane, and probing the membrane with a labeled cDNA? a) northern blot c) western blot b) Southern blot d ) eastern blot 8. What do we call a small nucleotide polymorphism that results in the production or elimination of a restriction endonuclease site? c) paralogue e) GFP b) contig d) RFLP 9. Coding DNA regions (exons) make up about what percentage of the human genome? c) 10% e) 75% b) 5% d) 25% a) 1%

Answer 1

1. b) defence against viral infections

Note: some bacteria normally produce endonucleases for defence against viral infections. They have a specific DNA sequence.

2. b) False

Note: some restriction enzyme acts as blunt cutters. They cut straight down the middle of a target DNA sequence without overhanging regions.

3. d) none of the above

Note: the number represent how long is the recognition site of enzyme. GAATTC is a 6 cutter.

4. c) polyA region

Note: the three essential components of a cloning vector are origin replication (ori), dominant selectable marker and polylinker region