1. E) Ligase catalyzes the joining of two terminals of DNA.
2. D) Vectors includes plasmids, bacterial artifical chromosome (BAC), yeast artificial chromosome (YAC), phage DNA and cosmids.
3. A) 3' overhang because cleaved single-stranded bases end with a 3' hydroxyl.
4. C) Restriction endonuclease recognize a specific palindromic sequence and cleaves it.
5. B) Reverse genetics. It starts from a protein/DNA with unknown function and then works backward to make a mutant gene, resulting in mutant phenotype.
6. C) BACs have the highest capacity (150-350 kbp) out of the given Vectors: plasmid (5 kbp), cosmid (35-45 kbp), bacteriophage lambda (35-50 kbp), phage p1 (75-100 kbp).
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called...
please answer all amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase d. Restriction endonuclease 47. Which of the following requires contact between a virus and a recipient bacterium for transfer of DNA? a. Crossing over b. Mutation c. Transduction d. Conjugation e. Transformation 48. In Recombinant DNA technology a vector is used to inserts the DNA into a host cell True/ False 49. If a foreign gene inserted into a plasmid inactivates the beta-galactosidase gene,...
Which of the following enzymes joins the paired sticky ends of DNA fragments? a. reverse transcriptase b. restriction enzymes c. DNA ligase d. DNA polymerase e. helicase
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA or plasmid D) pAMP
You wake up one morning to your roommate exclaiming about her sudden hair growth. She has been supplementing her diet with a strange new fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in the hope of cloning the harЕ gene. If you succeed you will be a...
4. Which form of DNA is possible as well as the linear form? a. circular b. stem loop c. A-DNA d. supercoiled e. B-DNA 5. Why might a single base-pair mutation in eukaryotic mRNA be less serious than one in prokaryotic mRNA? a. If the mutation occurs in the exon, it will not affect the gene product. b. If the mutation occurs in the 3′ end of the start site, it will not affect the gene product. c. If the...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
1 Integrase Doring En mapping 15 Wind D.Oligonucleotides. E Primers the followi N A from a single stranded RNA template D) Endonuclease 1 DNA polymer CRNA polymerase 25. A DNA ADNA quence of unknown function that could potentially be translated into protein is C Satellite DNA E ORI D. Contig 20.Overlapping clones wedi e mapping are called EDNA DI DNA 27 Which of the following vectors can accept the largest DNA fragments in cloning experiments? A Pesmid D. Cosmids E...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...