Question

1 Integrase Doring En mapping 15 Wind D.Oligonucleotides. E Primers the followi N A from a single stranded RNA template D) Endonuclease 1 DNA polymer CRNA polymerase 25. A DNA ADNA quence of unknown function that could potentially be translated into protein is C Satellite DNA E ORI D. Contig 20.Overlapping clones wedi e mapping are called EDNA DI DNA 27 Which of the following vectors can accept the largest DNA fragments in cloning experiments? A Pesmid D. Cosmids E Phage PI С ҮAcs 28 The cloning site that contains unique cleavage sites for many different restriction enzymes is called an) A Multivalent D Poly some E Polylinker 29. Sepuences identified from DNAs are called A Sequence tagged site Open reading frames CPR products D. Conti E. Expressed sequence tags 30. ARNA molecule looks like a compact when it is folded into its functional configuration DP

Answer 1

30) A tRNA molecules looks like a compact L ( L 5' ribozyme nucleotide) when it is folded into its functional configuration.

29) Sequence identified from cDNAs are called sequence tagged sites.

28) The cloning sites that contain cleavage sites for many different restriction enzyme is called a multiple cloning sites / polylinker.

27) Vector can accept the largest DNA fragments in cloning experinments are yeast artifical chromosomes(YACs).

26) Overlapping clones used in gene mapping are called contigs.

25) A DNA sequence of unknown function that could potentially be translated into proteins is ORFs.

24) Enzyme that synthesis DNA from a single stranded RNA template - helicase enzyme.

19) Plasmids that carry foreign genes are called vectors.

21) Restriction enzyme/ restriction endonuclease enzyme that clevage DNA at sequence specific sites.

22) The technique to look for possible gene functions by motating wild type genes is called reverse genetics.