Question

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amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase C Restriction cndonuclese D. Contig building E. Gene mapping 22. The technique to look for possible gene function by mutating wild-type genes is called A Gene therapy B Reverse genetics C. Transgenetics D. Oligonucleotides E. Primers 23. DNA temmini without overhangs produced by endonuclease digestion are called A. Cohesive termini B.Sticky ends C. Blunt ends D. Ligase E. Endonuclease 24 Which of the following enrymes synthesizes DNA from a single-stranded RNA template? A.Reverse transcriptase B. DNA polymerase C RNA polymerase 25, A DNA sequence of unknown function that could potentially be translated into protein is C. Satellite DNA E. ORF A eDNA B. BAC D. Contig 26. Overlapping clones used in gene mapping are called A. Contigs B. STS E. cDNAs C. ORFs D. T DNAs 27, Which of the following vectors can accept the largest DNA fragments in cloning experiments? A. Plasmid B. Bacteriophage lambda C. YACs D. Cosmids E. Phage P 28. The cloning site that contains unique cleavage sites for many different restriction enzymes is called a(n) A. Multivalent B. Enhancer trap C. Linkage group D. Polysome E. Polylinker 29. Sequences identified from eDNAs are called A. Sequence-tagged site B. Open reading frames C. PCR products D. Contigs E. Expressed sequence tags 30. A tRNA molecule looks like a compact when it is folded into its functional configuration. D. P C C. B. A G

Answer 1

QUESTION 19

The correct answer is Vector: A vector is a DNA molecule which is used as vehicle to carry a foreign genetic material into another cell (for example a plasmid). A vector that contains foreign DNA is defined as recombinant DNA. There are four major types of vectors:

Plasmids

Viral vectors

Cosmids

Artificial chromosomes.