Question

What is the dilution factor of the examples below:                         a) 20 uL serum added...

What is the dilution factor of the examples below:

           

            a) 20 uL serum added to 180 uL of saline

            b) 50 uL of serum added to 450 uL of saline

            c) 50 uL of serum added to 200 uL of saline

            d) 100 uL of serum added to 900 uL of saline

            e) 25 uL of serum added to 475 uL of saline

            f) 50 uL of serum added to 150 uL of saline

            g) 5 uL of serum added to 195 uL of saline

0 0
Add a comment Improve this question Transcribed image text
Answer #1

Formula for dilution factor, DF is:

DF = (Final volume) / (Solute volume)

(a): Volume of solute = 20 uL

Final volume = 20 uL + 180 uL = 200 uL

DF = (Final volume) / (Solute volume) = 200 uL / 20 uL = 10 (Answer)

(b): Volume of solute = 50 uL

Final volume = 50 uL + 450 uL = 500 uL

DF = (Final volume) / (Solute volume) = 500 uL / 50 uL = 10 (Answer)

(c): Volume of solute = 50 uL

Final volume = 50 uL + 200 uL = 250 uL

DF = (Final volume) / (Solute volume) = 250 uL / 50 uL = 5 (Answer)

(d): Volume of solute = 100 uL

Final volume = 100 uL + 900 uL = 1000 uL

DF = (Final volume) / (Solute volume) = 1000 uL / 100 uL = 10 (Answer)

(e): Volume of solute = 25 uL

Final volume = 25 uL + 475 uL = 500 uL

DF = (Final volume) / (Solute volume) = 500 uL / 25 uL = 20 (Answer)

(f): Volume of solute = 50 uL

Final volume = 50 uL + 150 uL = 200 uL

DF = (Final volume) / (Solute volume) = 200 uL / 50 uL = 4 (Answer)

(g): Volume of solute = 5 uL

Final volume = 5 uL + 195 uL = 200 uL

DF = (Final volume) / (Solute volume) = 200 uL / 5 uL = 40 (Answer)

Add a comment
Know the answer?
Add Answer to:
What is the dilution factor of the examples below:                         a) 20 uL serum added...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • 5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution...

    5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution Factor 10 (1:10 20 "uL 45 uL 50 ul 100 ul 150 uL 0.18 "mL" 0.090 mL 10 C. 0.400 mL 3

  • dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...

    dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...

  • Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...

    Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...

  • Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...

    Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...

  • Also— what is the dilution factor in tube 2? please show the steps to the answers!...

    Also— what is the dilution factor in tube 2? please show the steps to the answers! Practice Problems Solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample added to 200 mL diluent 4. 2 mL...

  • please do 5, 6, 7 and also please tell me the dilution factor of tube two...

    please do 5, 6, 7 and also please tell me the dilution factor of tube two (that is number 8 on a different page) Practice Problems solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample...

  • 1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...

    1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...

  • 1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32,...

    1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...

  • need help filling in the blanks of this table T Table Media Comment Insert Chart Text...

    need help filling in the blanks of this table T Table Media Comment Insert Chart Text Shape ONE) the initiai. SRprofit LBorofit Psr Pir TRsr TRIr Q TC 80 1 100 195 2 150 70 170 145 60 3 180 4 220 120 50 5 280 95 40 Top and bottom are separate examples. Circle the SR and LR level of output at the bottom answers:-80-20-20-10 001.75 1.75 222.0 3 3.50 80 80 95 100 140 175 180 190 195...

  • 1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...

    1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT