Homework Answers
Complete table
|
Patient sample (μl) |
Diluent (ml) |
Dilution factor |
|
|
20 |
0.18 |
10 |
|
|
a |
45 |
0.090 |
3 |
|
b |
50 |
0.450 |
10 |
|
c |
100 |
0.400 |
5 |
|
d |
150 |
0.300 |
3 |
By the formula DF= Vf/Vi
Where DF= dilution factor
Vf =final volume and Vi =initial volume
Add Answer to:
5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution...
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
please do 5, 6, 7 and also please tell me the dilution factor of tube two...
please do 5, 6, 7
and also please tell me the dilution factor of tube two (that
is number 8 on a different page)
Practice Problems solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Also— what is the dilution factor in tube 2? please show the steps to the answers!...
Also— what is the dilution factor in tube 2?
please show the steps to the answers!
Practice Problems Solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample added to 200 mL diluent 4. 2 mL...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
What is the dilution factor of the examples below: a) 20 uL serum added...
What is the dilution factor of the examples below: a) 20 uL serum added to 180 uL of saline b) 50 uL of serum added to 450 uL of saline c) 50 uL of serum added to 200 uL of saline d) 100 uL of serum added to 900 uL of saline e) 25 uL of serum added to 475 uL of saline f) 50 uL of serum added to 150 uL of...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
please do 5, 6, 7
and also please tell me the dilution factor of tube two (that
is number 8 on a different page)
Practice Problems solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Also— what is the dilution factor in tube 2?
please show the steps to the answers!
Practice Problems Solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample added to 200 mL diluent 4. 2 mL...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
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