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Please propose an experimental approach to generate a new form of chymotrypsin that is both acidophilic...

Please propose an experimental approach to generate a new form of chymotrypsin that is both acidophilic and psychrophilic.

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Acidophilic : ability of a substance to perform well in acidic condition and bad in basic condition.

Psychrophilic : ability of a substance to grow and reproduce in low temperature.

The New form of Chymotrypsin discussed here is : ( CHYMOTRYPSIN C ) This differs from the chymotrypsin A and B in its catalysing the hydrolysis of peptide and ester bonds at the carboxyl group of various amino acids .

Experimental approach : Here,Chymotrypsin C can be prepared from porcine pancreas glands by fractionation and chromatography on DEAE cellulose and inactive precursors of Chymotrpsin C is separated from the procarboxypeptidase A by gel filtration.

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