Question

In which of the following situations is a spectrophotometer a useful instrument? a. producing new substances d. microsurgery
b. producing high energy photons e. all of the above
c. detecting trace amounts of substances

Answer 1

Spectrophotometry is the technique where the intensity of incident light and the transmitted light is majored and compared with the standards. The amount of light absorbed gives a direct relation with the amount of substance-using Lambert-Beer's law. This method is sensitive enough for the detection of trace amount of substance as well.

Thus answer is option c) detecting trace amounts of substances.