Which groove (major or minor) contains more “information” and why? How do proteins interact specifically with this DNA groove, as when a zinc finger crosses the groove?
Where do you find A DNA, B DNA, Z DNA, and quadruplex DNA?
What contributes to DNA stability with regards to Tm? Which type of base pairs take the most energy to pull apart (i.e. require a higher temperature/Tm to dissociate
Define the following: negative supercoil, positive supercoil, topoisomerase I (note the role of tyrosine), topoisomerase II (note the role of ATP)
Answer
Major groove contains most information because the majority of the base pair is exposed more functional group because less backbone interference is there as compared to minor groove. So most of the protein binds with the major groove of DNA except few exceptions as major groove have more bases exposed which are available to interact with other molecules.
Which groove (major or minor) contains more “information” and why? How do proteins interact specifically with...
1. What was the significance of Photo 51? 2. What causes there to be a major and minor groove? What is the difference? 3. Which groove contains more “information” and why? How do proteins interact specifically with this DNA groove, as when a zinc finger crosses the groove? 4. Which of the following base pairs could proteins distinguish, and why? a. Major groove base pairs (GC/CG, AT/TA, GC/TA, GC/AT) b. Minor groove base pairs (GC/CG, AT/TA, GC/TA, GC/AT) 5. Where...