Question

Here is a DNA sequence obtained from sequencing.

1. Does this sequence code for a gene?  How can you tell?
2. Is this a eukaryotic or prokaryotic gene? How can you tell?
3. If it does code for a gene, what is the protein sequence encoded by this DNA sequence? Explain how you can tell.
4. Are there any regulatory regions encoded by this DNA sequence? How can you tell?TTATGTATGTAGATGGGGCAGCTAACAGGGAGACTAAATTAGGAAAAGCAGGTTATGTTACTGACAGAGGAAGACAAAAGGTTGTTTCCATAACTGACACAACAAATCAGAAGACAGAGTTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATCAGAAGTAAACATAGTAACAGACTCACAATATGCATTAGGGATCATTCAAGCACAACCAGATAAAAGTGAATCAGAGTTAGTTAGCCAAATAATAGAGCAGTTAATAAATAAGGAAAAGATCTACCTGGCATGGGTACCAGCACATAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTTAGTGCTGGAGTCAGGAAAGTATAGTTT

Answer 1

Answer:

1):

• Yes. The given sequence codes for a protein.
• It contains an open reading frame (A start and stop codon with intervening amino acid coding sequences).

2):

• Most likely, the given sequence codes for a prokaryotic protein as it does not contain any introns.

3):See image.

4):

• There are no regulatory elements in the given sequence.
• There is a 2 bp sequence upstream to the start codon and 3 bp sequence downstream to the stop codon.
• The remaining sequence is the ORF.

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