Question

Is more polar amino acid will have more affinity toward stationary or mobile phase .

Is less polar amino acid will have more affinity toward stationary or mobile phase.

Answer 1

Chromatography technique consists of a mobile phase and a stationary phase.

Mobile phase is the solvent which moves through the column and stationary phase is the substance which is imobile.

The components move on the basis of their adsorbing capacity through the column.

Separation of different components takes place by taking their solubility and adsorbing capacity into account.

The component having higher adsorbing capacity to the stationary phase move slower through the column.

The component which is soluble in the mobile phase move faster.

Suppose, in a column silica is the stationary phase which is polar in nature and hexane is the mobile phase which is non polar.

In that case, polar amino acid will have more affinity towards stationary phase and less polar amino acid will have affinity towards the mobile phase.