Question

Let's say you have a mixture for which you want to check the composition. Unfortunately, after...

Let's say you have a mixture for which you want to check the composition. Unfortunately, after quite a while, and several TLC analyses using our system, no spots have moved up the plate. What change could you make in the TLC system to try to get at least one compound in the original mixture to move up the plate?
1- try a more polar solvent
2- try a less polar solvent
3- try a more polar adsorbent

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Answer #1

The movement of compounds in TLC is depends on the binding interaction between adsorbent (stationary phase) and polarity of the solvent (mobile phase).

If the compound is more polar, we have to use more polar solvent to move the components in a mixture.

If the compound is less polar, we have to use less polar solvent to move the components in a mixture.

Therefore in the above case more polar sovent has to use to move the components in a mixture.

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