Question

Consider the enzyme-catalyzed reaction with Vmax=164 (μmol/L)min−1 and KM=32μmol/L. Part A If the total enzyme concentration...

Consider the enzyme-catalyzed reaction with Vmax=164 (μmol/L)min−1 and KM=32μmol/L.

Part A

If the total enzyme concentration was 6 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute?

Express your answer to three significant figures.

kcat

kcat

=
2.73×104   min−1  

Part B

Calculate kcat/KM for the enzyme reaction.

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