Question

In cation exchange chromatography, the beads are covalently linked to a ________chemical and a ______________ ion is used as the counter-ion.

a. positive, negative

b. negative, positive

c. negative, negative

d. positive, positive

Which of the following is NOT an application of a DNA microarray?

a. SNP detection

b. comparative genomic hybridization

c. gene expression profiling

d. alternative splicing detection

e. imprinting

Individuals susceptible to tumorigenesis have an abundance of receptors specific for:

a. serotonin

b. testosterone

c. acetylcholine

d. epinephrine

e. estrogen

The signaling receptors commonly found in neuromuscular junctions are :

a. ligand-gated ion channel receptors

b. nuclear hormone receptors

c. G-protein coupled receptors (GPCRs)

d. receptor tyrosine kinases (RTKs)

e. none of the above

Answer 1

QUESTION 1

In cation exchange chromatography, the beads are covalently linked to a negative chemical and a positive ion is used as the counter-ion.

The cation exchange chromatography is based on a negatively charged resin (or beads in this case) that will allow the attachment of positively charged proteins (remember that opposite charges attract each other). The resin or bead will be the stationary phase while the protein sample will be the mobile phase.

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