Question

1. Staurosporine (FW: 466.53 g/mol) is a potent reagent used to inhibit various PKC isoforms in human cell lines, and is supplied in a vial containing 2 mg of lyophilized powder. You want to treat 10 ml of HL-60 cells with a final concentration of 10ηM (nanoMolar) staurosporine, and you only want to add 5 μl of staurosporine solution to the cells. What concentration should you make your stock solution of staurosporine in order to make this 5 µl addition? Based on this concentration, how would you make the staurosporine stock solution using DMSO as a solvent?

Answer 1