Question

help with these four please w/ an explanation

T/F: Centrifugation can only be used to separate large objects, but small particles like microsomes and ribosomes cannot be separated.

T/F: A disaccharide consists of a sugar covalently linked to another molecule such as an amino acid or a nucleotide

T/F: The hydroxyl groups on monosaccharides are reaction hotspots and can be replaced by other functional groups to produce derivatives of the original sugar.

T/F: After protein separation by SDS-PAGE each band on the gel represents only one protein

Answer 1

1. False

Centrifugation is a technique that is able separate organelles on the basis of their density. It can separate each and every organelle, be it mitochondria chloroplast, ribosomes, lysosomes, golgi and others on the basis of their density. Organelles arrange themselves in the centrifuge tube on the basis of their density, organelles with lowest density occupy the upper part of tube and those with higher density occupy lower part of the tube.

2. False

Dissacharide bond is formed by covalent linkage or gkycosidic linkage between 2 monosaccharides only.

3. True

Hudroxyl group on monosaccharides can be replaced with amine, sulphur, phosphate, carboxyl group to form sugar amine, sugar sulphate, sugar phosphate, sugar acid. There are hundreds of derivatives possible like these.

4. True

Each band on a gel represents one type of protein only. One band can have many molecules of that protein which is proportional to the intensity of band. Higher the number of protein molecules, darker will be the colour of the band. If a protein contains 3 polypeptide chain, then you will see 3 bands for this protein on the gel.

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