Question

Answer 1

σ⁷⁰(RpoD) – σ^A – the "housekeeping" sigma factor or also called as primary sigma factor (Group 1), transcribes most genes in growing cells. Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating.^[1] In the case of E. coli and other gram-negative rod-shaped bacteria, the "housekeeping" sigma factor is σ⁷⁰.

6.1 The position of sigma70 will be upstream to the promoter region. The sigma subunit binds with the RNA core polymerase. This considerably decreases the affinity of the enzyme for general regions of the DNA strand, and recognizes and binds t the promoter site of the coding region of the template strand.

6.2

3'UTE

The transcript is mRNA produced by transcription. ORF is the open reading frame which the sequence of codons on mRNA for the translation into protein.

6.3 3'-UTR region is the untranslated region just immediately after the stop codon till poly A tail, on mRNA. This is not translated into protein.

6.4 5'-UTR region is directly upstream to the initiation codon AUG. This is also not translated into protein.

6.5 viii - 3'uccacca 5' near the 3' end of 16S molecule.

Shine- Dalgamo has the sequence 5' -agguggu-3' which will pair with the 3' end of 16S rRNA.