Question

B. Suppose ES cells are obtained from a grey fur mouse, transfected with an engineered plasmid construct and cells undergoing homologous recombination were selected. These engineered ES cells were then injected into the blastocysts obtained from a white fur mouse and implanted into a surrogate mother What types of fur colors are expected in pups developed from these implanted blastocysts and why?

Answer 1

Chimaera pups will be obtained which will have some regions with grey color and some with white color.

As both types of ES cells are there in blastocyst so genetically distinct cell lineages will be formed.