The order of amino acids (Lys, Asp, ILE) eluted from an anion exchange column at PH 7 should be:
KDI
DIK
DKI
IKD
no correct answer
Please explain why the answer is no correct answer. Thank you.
The order of amino acids (Lys, Asp, ILE) eluted from an anion exchange column at PH 7 should be: KDI DIK DKI IKD no correct answer Please explain why the answer is no correct answer. Thank you.
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala plus lys. anion exchange resin was used Tubes 2,3, 6 and 7 had a spot appear that was light pink We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Please answer thoroughly, will rate thumbs up. thanks Q1. Consider the following protein sequence: Leu-Lys-Val-Asp-Ile-Ser-Leu-Arg-Leu-Lys-Ile-Arg-Phe-Glu. What is special about the arrangement of the amino acids in these sequences when incorporated into a Beta sheet? What prediction can you make about how this Beta sheet might be arranged in a cytoplasmic protein. Q2. Consider the following protein sequence as an Alpha-helix: Leu-Lys-Arg-Ile-Val-Asp-Ile-Leu-Ser-Arg-Leu-Phe-Lys-Val. What is special about the arrangement of the amino acids in these sequences when folded into alpha helix?
Predict the charge on the major species of the peptide Ile-Lys-Asp-Arg-Ser-Gln at pH 7.0 Please explain if you can. Thanks
3. A protein contains the following amino acids: O ALA 4 GLN 1 LEU 3 ARG 4 GLU 4 LYS 4 ASN 5 GLY O MET 1 ASP 1 HIS 2 PHE 4 ILE 4 PRO 8 SER 5 THR 1 TRP 2 TYR 2 VAL BGYS. a) What is its net charge at pH 1? b) What is its net charge at PH 13? c) Calculate the pl. 4. In what order would the amino acids GLU, LYS, and...
What is the overall charge for this pentapeptide Leu-Met-Lys-Arg at pH 7? will it bind to an anion exchange column at this pH? please explain the process thank you
)The enzyme Rubisco contains a reaction center that involves the side chains of four key amino acids, Lys201, Asp223, Glu246, His294. These occur in that order in the primary sequence, but they are not directly connected. Use the outline to draw in the correct structures for each of the amino acids (do not need to include peptide bonds). Use the correct ionized state for each of them at pH 7. (pKa values for side chains of Lys, Asp, Glu, His...
The overall charge of the polypeptide His-Tyr-Lys-Met-Ile-Glu at pH 6.9 is __? Please explain how you got the answer.
Can you clearly explain the order of elution and exactly WHY they elute in that specific order. 8. Using a cation exchange resin, a mixture of 3 amino acids-- Arg, Asp, and Ser - is separated using an elution gradient of increasing NaCI solution. What would be the correct elution sequence?
#2 is Q HiTrap column where it is anion exchange column not cation 2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
The chemical structures of the 20 standard amino acids at pH 7, along with their 3 and 1 letter codes, are givern in alphabetical order below. While the oxygen and nitrogen atoms of the peptide bonds may serve as a donor atom to complex a metal ion, very often the ligands for the metal come from the amino acid side chains. Takea few moments to examine the chemical structures of the different side chains. Circle the side chains that you...